Five pregnant women joined with immigrant rights groups to file a lawsuit in federal court Wednesday challenging President Donald Trump’s executive order that seeks to overturn birthright citizenship.

The lawsuit accused Trump’s executive order, “Protecting the Meaning and Value of American Citizenship,” of trying to end the constitutional right of birthright citizenship protected in the Fourteenth Amendment.

The lawsuit is led by CASA, a national organization serving working-class Black, Latino, African descendant, Indigenous, and immigrant communities; ASAP, which provides asylum seekers with legal and community support; and the Institute for Constitutional Advocacy and Protection at Georgetown Law, which represents one of the women.

Trump’s executive order aims to eliminate the 14th Amendment’s guarantee that “All persons born or naturalized in the United States, and subject to the jurisdiction thereof, are citizens of the United States and of the State wherein they reside,” according to a release by the plaintiffs.

“Many ASAP and CASA members will certainly be impacted by this order. There is a huge question mark for thousands of people,” said Conchita Cruz, co-founder and co-executive director of ASAP.

Trump’s order “undermines the very fabric of our democracy and represents a direct assault on the constitution,” said Nicholas Katz, CASA’s general counsel in a written statement.

“By attempting to deny U.S. citizenship to children born within our borders, based solely on the immigration status of their parents, President Trump is seeking to upend centuries of history and established legal protections through executive fiat. This will not stand,” Katz added.

Three of the women are from Hyattsville. The other two are from New York City, according to court documents.

At the press conference Wednesday, a plaintiff named Monica said she is a medical doctor from Venezuela who is 12 weeks pregnant. She said she is seeking asylum with temporary protected status. (Monica declined to provide her last name, citing fear of retaliation and safety.)

Since moving to the United States in 2019, Monica said she and her husband have been working, paying taxes and have even been able to buy a home.

“After six years living in the United States, my husband and I wanted to become parents,” she said through a translator. “I should be worried about the health of my child. Instead, my husband and I are stressed and depressed.

“I am shocked that the government does not want my child born in the United States to have U.S. citizenship,” she added. “I am scared for my child and for our family, especially since we cannot return to our country or get our child any other citizenship — I don’t know what we will do,” Monica said.

Trump, his federal Department of Homeland Security, the Secretary of State and Attorney General, who are all listed as defendants in the lawsuit, could not immediately be reached for comment.